results our study showed that the sensitivity and specificity of taqman real time pcr was 96% and 95% respectively. these values were 89% and 90, 38% and 100%, 6% and 100% for pcr, culture and microscopy, respectively. conclusions our study showed that sensitivity of taqman real time pcr was higher that pcr, culture and microscopy but specificity of culture and microscopy was more than pcr and ...

dear editor-in-chief we read with interest the study by khazaei et al. (1) in which the authors have nicely concluded that pcr is more sensitive test than ziehl-neelsen staining and histo-pathological examination for the diagnosis of tuberculosis (tb). they have rightly pointed to use pcr, selectively, in acidfast bacilli negative paucibacillary forms of tb. however, we intend to highlight few ...

background neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years, and it is characterized by wide clinical and biological heterogeneity. n-myc oncogene amplification is considered to be one of the most important prognostic factors used to evaluate survival in these patients. objectives the aim of our study was to determine amplification of the n-myc oncog...

results 162 yeast species from 250 specimens were isolated in sabroud dextrose agar (sda) medium. 106 (65.4%) of them were germ tube formation positive, 86 (53%) chlamydoconidium formation positive and 101(62.4%) were pcr positive. yeast cells and mycelia were detected in the isolates on direct microscopic examination. c. albicans accounted for 66.04% of cases and 34% were non-c. albicans speci...

results all eight candida species were successfully detected, identified and quantitated based on the icl gene. a seven-log range of the gene copy number and a minimum detection limit of 103 copies were achieved. conclusions a one-tube absolute quantification real-time pcr that differentiates medically important candida species via individual unique melting temperature was achieved. analytical ...

background aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). objectives this study aimed to evaluate the frequency of aseptic meningitis caused by herpesviruses, namely herpes simplex types i and ii (hsv-1, hsv-2), epstein-barr virus (ebv), cytomegalovirus (...

conclusions in the present study, the highest prevalence was for st5, which is so important for epidemiology and risk of human infection. the report related to st3 in cattle as a subtype of human showed mutual infection between human and cattle. another important point in this study was the st6 report. finally, it seems that gathering epidemiological data is needed for a better understanding of...

background toxoplasma gondii is an obligate, intracellular parasite, which is widely spread in the world. the parasite is able to infect all warm-blooded hosts including human.the infection occures via consumption of food or water containing oocytes, eatingundercooked meats containing tissue cysts, and placenta. undercooked meat consumption is one of the most important ways of toxoplasma transm...

conclusions using the pcr-rflp method, three distinctive h. pylori caga genotypes were detected in antral biopsies. genotype i, which was predominant among the isolates, was significantly associated with gastritis. however, the data showed that caga genotype iii may play a role in duodenitis and duodenal ulcers in patients infected with h. pylori. patients and methods a total of 161 gastric bio...